This study aims to model the most common female endocrinopathy, Polycystic Ovary 

Syndrome (PCOS), in Drosophila melanogaster. If insulin-resistant flies are put on high 

glucose diets combined with excessive androgen levels, then Metformin, Clomiphene 

Citrate, and RNAi will lessen polycystic symptoms. PCOS symptoms were induced into 

insulin-resistant flies by adding 20-hydroxyecdysone to medium to create 

hyperandrogenism in the flies, and increasing fructose intake leading to obesity and 

hyperglycemia. The effects of Metformin and Clomiphene citrate, common drug 

treatments for PCOS, were observed on hemolymph glucose concentration and 

fecundity; to test insulin functionality, an insulin sensitivity assay was run. After 

induction of PCOS, the DENND1A gene was knocked out using RNAi plasmid 

microinjections to observe the function of a human locus in drosophila. To reinforce 

successful intake of plasmid dsRNA, a PCR gel was run using primers. Another tested 

assay was immunofluorescence microscopy on drosophila ovaries. Drosophila were 

embedded, sectioned, and stained with antibodies to observe the anti-EcR receptor 

which codes for ecdysone activity, which control ovarian maturation in insects. Overall, 

results supported that a polycystic-like disorder can be induced in flies, 

hyperandrogenism and hyperglycemia did not significantly increase ovarian ecdysone 

activity, and DENND1A function was not significantly reported in fecundity. These 

results can be applied to finding novel and cheap methods to research PCOS, as well 

as how genetics of the disease function in drosophila. More testing can be done on 

environmental factors that may affect genetic factors in certain individuals that make 

them prone to certain symptoms.